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Site-specific placement of unnatural amino acids, particularly those responsive to light, offers an elegant approach to control protein function and capture their fleeting ‘interactome’. Herein, we have resurrected 4-(trifluoromethyldiazirinyl)-phenylalanine, an underutilized photo-crosslinker, by introducing several key features including easy synthetic access, site-specific incorporation by ‘privileged’ synthetases and superior crosslinking efficiency, to develop photo-crosslinkable bromodomains suitable for ‘interactome’ profiling.more » « less
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Kuwik, Jordan; Hinkelman, Kathryn; Waldman, Megan; Stepler, Kaitlyn E.; Wagner, Shana; Arora, Simran; Chernenkoff, Sasha; Cabalteja, Chino; Sidoli, Simone; Robinson, Renã AS; et al (, Journal of the American Chemical Society)
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Wagner, Shana; Waldman, Megan; Arora, Simran; Wang, Sinan; Scott, Valerie; Islam, Kabirul (, ChemBioChem)
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